New 12-hour Salmonella test
A 12-hour test for detecting salmonella in poultry meat
has been developed by Danish researchers.
can take up to 5 days before results are known.
Real-time PCR technology in recent results has shown to offer several advantages
in detecting salmonella.
Researchers in this latest study developed a 12-hour DNA-based method for
detecting salmonella bacteria using polymerase chain reaction (PCR). This test
was developed based on eight hours of pre-enrichment, followed by automated DNA
extraction and a sensitive real-time PCR. The method, published by the American Society for
, attempts to yield the highest amount of cells and
Next, results were compared to a reference culture method, which previously
tested 100 minced meat and poultry samples following 24 hours of pre-enrichment
and showed relative accuracy and sensitivity of 99% and specificity of
"It was successfully demonstrated that the optimized 12-hour PCR method for
Salmonella detection produced results comparable to those of the reference
culture method with artificially inoculated pork meat and poultry samples," say
the researchers. "The main advantage of the method developed is the reduced time
of analysis, enabling faster release of Salmonella-free fresh meat."
(M.H. Josefsen, M. Krause, F. Hansen, J. Hoorfar. 2007. Optimization of
a 12-hour TaqMan PCR-based method for detection of salmonella bacteria in meat.
Applied and Environmental Microbiology, 73. 9: 3040-3048).
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