Background 2776 views update:Mar 9, 2016

Functional additives as an alternative to AGP's

Global poultry production is expected to increase 5% per year. At the same time consumers demand healthier safety-proven production methods. The trend to reduce antibiotic uses is undeniable, and is steadily expanding. Simultaneously, the increasing ineffectiveness of antibiotics due to resistance 
development is another concern.

The concern about antibiotic residues in meat products is solved in many countries, but the bacterial resistance to antibiotics is a much more complex problem, involving the entire medical profession. In animal production—poultry production in particular—ceasing the use of antibiotics as growth promoters has changed the technical approaches used to counter the removal or reduction of these products. Without antibiotics, the microbiota in the birds’ gastrointestinal tract must be viewed as an entity to implant, develop and control to ensure that the animals are healthy and can grow according to their genetic potential.

Antibiotic resistance and animal production

To understand antibiotic resistance, it is necessary to differentiate natural from acquired resistance. Regardless of the antibiotic molecule, resistant bacteria pre-exist. This means that an antibiotic doesn’t have an effect on all bacteria. Some may be beyond the molecule’s spectrum of action.

Acquired resistance has never rendered a species of bacteria completely resistant. In acquired resistance, the bacteria develop mechanisms that allow them to survive. In the presence of a certain concentration of antibiotics, some bacteria die, but others do not, as they already have a certain resistance to the antibiotic. In concrete terms, acquired resistance either results from a mutation of the bacterium’s chromosome (making it less sensitive to the antibiotic), or, more often, the acquisition of a gene from a foreign “donor” bacteria, thus transferring resistance horizontally.

Whether due to natural or acquired resistance, the antibiotic no longer has the same effect on the resistant bacteria. Either the bacteria can destroy the antibiotic or render it inactive with an enzyme “coat” to prevent the active matter from entering or by expelling the matter as soon as it enters the cell. If an antibiotic is administered, a selection of the resistant bacteria occur and their multiplication allows the shift on the microbiota favouring only resistant bacteria. Removing antibiotic growth promoters from animal production is only part of the solution. Besides that adaptations had to be made to maintain production at optimal levels while preserving animal welfare disease control.

Intestinal health and intestinal microbiota

Removal of antibiotic growth promoters has revealed that it is crucial to manage the makeup of the birds’ intestinal microbiota to avoid or at least limit the risks of health problems inherent to intensive production. The main strategies can be outlined as follows: Selectively introduce favourable bacterial populations from a very young age; Provide a regular supply of nutrients specific to the beneficial bacteria; Act directly on pathogenic bacteria.

Different methods are available, such as the dietary supplementation with probiotics, prebiotics, organic acids and essential oils. These products act either avoiding bacterial adhesion to the intestinal cells or through bactericidal or bacteriostatic effects. The goal is to achieve the most stable balance on the microbiota in order to avoid the trouble caused by bacteria like E. coli, Clostridium and Salmonella. The gastrointestinal microbiota profile depends on numerous factors such as the health of the breeding stock, production system, stressors (vaccination, viral episodes, etc.), nutrition and stocking densities.

Bacteria can acquire resistance to antibiotics or have a natural resistance.

Selectively introducing favourable bacterial populations

The first bacteria to colonise the animals’ digestive tract will shape the intestinal ecosystem for the introduction of global intestinal microbiota. Selectively colonising the gastointestinal tract with beneficial bacteria can modulate the expression of certain genes in the tract’s epithelium, creating conditions for establishing beneficial microbiota. Since the first micro-organisms that come into contact with a newly hatched chick’s gastrointestinal tract are from the breeding stock, controlling the microbiota in the parents would be ideal. Studies have demonstrated that the use of beneficial bacteria in low doses in the hatchery improves chicks’ resistance to pathogens. Other studies have validated that an in-ovo injection of FOS (fructooligosaccharide) helps maintaining higher levels of bifidobacters, with positive effects on zootechnical performance and mortality rate.

Most of the microbial strains in probiotics are of the genus Bifidobacterium or Lactobacillus. In certain conditions, lactobacilli can produce metabolites that limit the growth of Salmonella by modulating immunity and avoiding bacterial binding to the epithelial cells of the intestine. However, according to some publications, this type of effect is limited.

Providing a regular supply of specific substrates

To reinforce the colonisation of beneficial bacteria, it is important to support their ability to flourish and compete with pathogens. Prebiotics are used in many cases as an additive to the feed. While the birds are unable to digest the prebiotics, they have a beneficial effect on the animals’ intestinal health by specifically stimulating the growth or activity of a limited number of favourable species of bacteria. Almost all prebiotics are oligosaccharides or polysaccharides, like FOS, several studies have shown their beneficial effect, as well as decreasing E. Coli and Clostridium populations in chicken caeca.

Enzymes are important additives specifically for energy and protein optimisation. They are also cited for their indirect effect on the development of beneficial microbiota. Research demonstrated that supplementing xylanase of bacterial origin to chickens infected with Salmonella sp during three days reduced carcass contamination on slaughter (-2 log CFU/g). By acting on the arabinoxylan, the xylanase plays a prebiotic role on the beneficial microbiota in the lower intestine.

Essential oils are extracts from plants that contain a large quantity of aromatic molecules in their fruits, seeds, bark or roots, which make up the plant’s active principles. Essential oils can have varied effects, such as antifungal, antiphrastic, antibacterial, anti-inflammatory. The review by Burt presents the antibacterial mechanism of action by targeting the pathogens’ membrane. Essential oils are mainly used to stabilise the animals’ digestive flora. The goal is to act on certain bacterial populations to obtain a more favourable profile for the animal.

It has also been demonstrated that coated products are far superior to non-coated products. This is because coated products are released much farther along the digestive tract, while non-coated products are absorbed or dissociated rapidly and will have diminished action in the intestine. Others demonstrated that associations between organic acids and essential oils can be effective against pathogens in the gastrointestinal tract. By using a combination of micro-encapsulated, essential oils and organic acids, or a blend of protected organic acid salts, Gauthier obtained positive results on chickens exposed to a necrotic enteritis challenge.

The effect on zootechnical performance of the combination of protected, organic acids and essential oils can be explained not only by the control of Clostridium and E. coli populations, but also by the decrease in virulence expression of E. coli populations in the chickens’ gastrointestinal tract. This approach is important for the safety of the finished product.

Viable alternative

In poultry production, interactions between the birds’ feed and their intestinal health have been amply demonstrated. In the past, the use of antibiotic growth enhancers had the potential to mask a number of problems. In the field, functional diet-based strategies need to be adapted to the different sanitary and production contexts for each production system. Selectively introducing favourable bacterial populations for young chicks, providing a regular supply of nutritious substrates specific to the beneficial bacteria and effectively controlling pathogenic bacteria are important courses of action to ensure the intestinal health of the birds via their feed. Prebiotics, enzymes, and combinations of organic acids and essential oils can be viable alternatives to antimicrobials.

References available upon request.

Jean-Christophe Bodin, innovation 
and development director Jefo

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